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Figure 3. Thapsigargin (TG) induces clusterin (CLU) promoter activity. Log-phase 1403 MCF-7 cells, which contain a stably integrated CLU promoter–firefly luciferase reporter construct [15], were exposed to various doses of TG (0–12 nM) and monitored for changes in CLU promoter activity at 24 h, 48 h, 72 h and 96 h post treatment. CLU promoter activities were monitored by standard luminometer assays, as described previously [15, 16]. Cells harvested at 24 h, 48 h, 72 h and 96 h after 10 Gy ionising radiation were used as a positive control. Experiments were performed three times in triplicate, and statistical analyses of the data were performed using the Student's t-test. NT, no treatment.
