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Figure 2. Different double strand break(DSB)-inducing activity in medium recipient cells as a function of time after irradiation. The conditioned medium harvested from the irradiated culture induced a significant excess of DSB-positive cells and showed time dependence. The irradiated medium without cells did not show notable DSB-inducing activity. Data were pooled from three or four individual experiments. Error bars represent the standard deviations of the means. The symbols * and ** depict values that are statistically significant (p<0.05 and p<0.01, respectively) between the corresponding control and experimental groups.