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Figure 4. (a,b) Subepithelial myofibroblasts derived from normal ileum and from radiation enteropathy were incubated with Y-27632 for 24 h (10, 100 µM). Subsequent fluorescein isothiocyanate (FITC) phalloidin staining showed alteration of the actin stress fibre network. (c,d) Rho kinase inhibition decreased connective tissue growth factor (CTGF) and COL1A1 (collagen Type 1 alpha 1) mRNA level, as assessed by real-time reverse transcriptase polymerase chain reaction (RT-PCR). Values are normalized to the level of 18S RNA.
