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Figure 2. A Plexiglas® polymethylmethacrylate block (thick black outline) served as a "saddle" for the mouse, viewed as it would be by an observer directly above it. The mouse was anaesthetized and placed prone on the block for its first tumour irradiation. In this figure, the outline of the mouse is represented by an ellipse. Two black dots represent its eyes. To avoid irradiating its left foreleg, the 150 mm long axis of the block was rotated 5° (about a vertical axis through the centre of the block) clockwise from the reference 0° microbeam direction. The microbeam array, symbolized by thin arrows, was propagated in a thin, wide, slightly divergent fan-beam, substantially in a horizontal plane, represented here as the plane of this page. The second irradiation was implemented after the block was rotated 95° clockwise from the 0° reference direction about the same vertical axis.