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Figure 3. Upper panels show pseudocolour images of tissue blood flow, in ml blood per gram tissue per minute (ml g–1 min–1), computed from autoradiograms of 20 µm thick frozen sections of 
15 mm diameter P22 rat tumours. Tumours were excised from an untreated rat (a) or at various times after treatment from rats treated with a clinically relevant dose of 10 mg kg–1 CA-4-P (b–d). Blood flow was computed pixel by pixel using the methods described in Figure 2. Lower panels show paraffin-embedded 3 µm thick tumour sections stained with haematoxylin and eosin from a separate group of rats treated as for the upper panel. Images in (a) show typical heterogeneity of blood flow in untreated tumours and spindle-shaped tumour cells in this sarcoma. Images in (b) show severely reduced blood flow accompanied by tumour cell shape changes and some dilated vessels and coagulation. Images in (c) show sustained blood flow reduction, more dilated, congested and coagulated vessels and extensive haemorrhage. The shaded areas in the upper panel indicate the extent of haemorrhage. Images in (d) show extensive necrosis (delineated in the upper panel) accompanied by very low blood flow, surrounded by a regrowing rim of tumour with flow values similar to controls. Figure adapted from reference [80]. Arrows indicate coagulation.
