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Figure 6. Iohexol promotes mitochondrial depolarization. Neutrophils were cultured in Rosweli Park Memorial Institute 1640 medium containing (a) 10% platelet poor plasma alone and (b) treated with iohexol 40 mg I ml-1. Mitochondrial transmembrane potential was measured using the styryl dye, JC-1, at 4 h, 10 h and 18 h in culture. Iohexol promotes a greater reduction in mitochondrial transmembrane potential compared with the control as assessed by increased formation of monomers (488nm-FL1-H). This effect was evident within 4 h of culture, with progressive loss of mitochondrial transmembrane potential at 10 h and almost complete mitochondrial depolarization at 18 h. Results representative of three experiments.
