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Figure 4. Effects of physicochemical properties of iodinated contrast media and growth factor dilution on neutrophil apoptosis. (a) Neutrophils were treated with experimental solutions for 12 h and apoptosis quantified by flow cytometry. Control, Rosweli Park Memorial Institute 1640 medium (RPMI)+10% platelet poor plasma; diatrizoate, 20 mg I ml-1; NaCl hypertonic, mannitol, and urea at same hyperosmolality as 20 mg I ml-1 diatrizoate; disodium calcium edetate (EDTA) 0.1 mg ml-1, 0.9% NaCl, RPMI diluted 1:2 with isotonic NaCl solution. Results expressed as mean percentage of apoptotic cells±standard error of the mean; 
p<0.01 for treated vs control neutrophils. (b) Neutrophils were cultured in RPMI containing 10% platelet poor plasma alone and treated with increasing concentrations (0.1–80 mg I ml-1) of povidone-iodine. Results expressed as mean percentage of necrotic cells±standard error of the mean; n=3–4; p<0.01 for treated vs control neutrophils.
