BJR
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
British Journal of Radiology (2007) 80, S7-S12
© 2007 British Institute of Radiology
doi: 10.1259/bjr/44550200
This Article
Right arrow Abstract Freely available
Right arrow Full Text
Right arrow Full Text (PDF)
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Han, W
Right arrow Articles by Yu, Z
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Han, W
Right arrow Articles by Yu, Z

The early and initiation processes of radiation-induced bystander effects involved in the induction of DNA double strand breaks in non-irradiated cultures

W Han, PhD L Wu, PhD B Hu, PhD L Zhang, MSc S Chen, MSc L Bao, BSc Y Zhao, BSc A Xu, PhD and Z Yu, BSc

Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, Anhui 230031, People's Republic of China


Figure 1
View larger version (15K):
[in this window]
[in a new window]

  		Figure 1. Bystander double strand break(DSB) induction response as a function of time in primary human fibroblasts. The fractions of DSB-positive cells were calculated 2.5, 5, 10, 15, 30, 40 and 60 min after irradiation with 1 cGy of {alpha}-particles. Data were pooled from three or four individual experiments. Error bars represent the standard deviations of the means. The symbols * and ** depict values that are statistically significant (p<0.05 and p<0.01, respectively) between the corresponding control and experimental groups.

 

Figure 2
View larger version (17K):
[in this window]
[in a new window]

  		Figure 2. Different double strand break(DSB)-inducing activity in medium recipient cells as a function of time after irradiation. The conditioned medium harvested from the irradiated culture induced a significant excess of DSB-positive cells and showed time dependence. The irradiated medium without cells did not show notable DSB-inducing activity. Data were pooled from three or four individual experiments. Error bars represent the standard deviations of the means. The symbols * and ** depict values that are statistically significant (p<0.05 and p<0.01, respectively) between the corresponding control and experimental groups.

 

Figure 3
View larger version (32K):
[in this window]
[in a new window]

  		Figure 3. The maximum induction of double strand breaks(DSBs) 30 min after irradiation with 1 cGy of {alpha}-particles in irradiated regions, non-irradiated regions and medium receptor cells. Treatment with or without 1% dimethyl sulphoxide (DMSO) showed that the free radical scavenger, DMSO, effectively inhibited the bystander response of DSB induction for all the groups. Data were pooled from three or four individual experiments. Error bars represent the standard deviations of the means. The symbol * depicts values that are statistically significant (p<0.01) between the DMSO-treated and untreated groups.

 




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
BJR DMFR IMAGING  ALL BIR JOURNALS 
Copyright © 2007 by the British Institute of Radiology.