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Iosimenol, a new non-ionic dimeric contrast medium, does not induce immunoreactivity in the popliteal lymph node assay

Departments of ¹ Biochemistry, ² Pharmacology, ³ Medical Microbiology and ⁴ Radiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, PO Box 17666, Tawam Street, Al Ain, Abu Dhabi, UAE

View larger version (16K): [in this window] [in a new window]   Figure 1. Flow cytometric analysis of PLN cells following treatment. Mice were given a single s.c. injection of 50µl of iosimenol (a), iodixanol (b), or STZ (c) in the right hind footpad. As a control, an equal volume of saline or citrate buffer was injected into the left hind footpad. On day 5 post injection, PLNs were collected, pooled and processed for flow cytometric analysis. Cells were double-stained with monoclonal antibodies specific to the following proteins: CD45/B220 (total B cells), CD3 and TcR C chain (total T cells), CD4 (T helper cells), CD8 (T cytotoxic cells) and F4/80 (macrophages). Each datum point represents the mean±SEM of five determinations from three separate experiments (iosimenol and iodixanol groups) or three determinations from two individual experiments (STZ group). Asterisks denote statistically significant differences from respective cell types in control-treated PLNs (*p<0.05, **p<0.01). Data from a total of 30 000 cells per group were collected and analysed by CELLQUEST.

View larger version (27K): [in this window] [in a new window]   Figure 2. Upregulation of cellular activation markers following treatment. The extent of activation of B and T lymphocytes was monitored by the level of expression of Ly6A/E (Sca-1) protein on the cell surface. (a) Pooled PLN cells were double-stained with a pair of direct conjugated monoclonal antibodies to B220 and Sca-1 (to identify activated B cells) or TcR C chain and Sca-1 (to identify activated T cells). The per cent Sca-1 positive B and T lymphocytes is shown in the bar graph. Each datum point represents the mean±SEM of three groups of pooled PLN cells from nine mice (iosimenol and iodixanol groups) or six mice (STZ group). Asterisks denote statistically significant differences from control (***p<0.001). (b) Representative dot blot profiles of PLN cells from the various treatment groups after staining with antibodies to B220 and Sca-1 antigens. Panels I to IV represent the staining profiles of PLN cells from control, STZ, iosimenol or iodixanol treated groups, respectively. The number shown in each of the quadrants denotes the percentage of cells in that quadrant. The dot blots represent analysis of one pooled PLN cells from each treatment group. Data from a total of 30 000 cells per group were collected and analysed by CELLQUEST. The data are representative of three (iosimenol and iodixanol groups) or two (STZ group) independent experiments.