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British Journal of Radiology (2005) 78, 976-988
© 2005 British Institute of Radiology
doi: 10.1259/bjr/55735832

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Optical coherence tomography

A Gh Podoleanu, PhD

School of Physical Sciences, University of Kent, Canterbury CT2 7NR, UK



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Figure 1. Cross section optical-coherence tomography (OCT) image from the skin on the human finger tip. Image obtained in 0.5 s using a superluminescent diode of central wavelength 793 nm and delivering 600 µW optical power to skin. Depth resolution is 15 µm.

 


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Figure 2. Optical-coherence tomography set-up. (a) Michelson interferometer; (b) in-fibre equivalent of the configuration in (a); OS, optical source; BS, beam-splitter; OUT, object under test; DC, single mode directional coupler.

 


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Figure 3. Comparison between the photodetector output in Figure 2Go when an ideal laser (top) or a broadband source (bottom) is used. OPD, optical path difference.

 


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Figure 4. Superposition of two wavetrains (interference of two beams generated by a source with a large bandwidth).

 


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Figure 5. Photodetected optical-coherence tomography signal (middle) for an object made of two interfaces (top) and the rectified output (bottom). Moving the reference mirror with velocity v, generates a time varying signal with frequency f=2v/{lambda}, where {lambda} is the central wavelength of the optical source.

 


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Figure 6. Relative orientation of the axial scan (A-scan), en face scan (T-scan), longitudinal slice (B-scan) and en face or transverse slice (C-scan).

 


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Figure 7. Different modes of operation of the three scanners in a flying spot optical-coherence tomography system.

 


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Figure 8. An in-fibre interferometer equipped with all three scanners for time domain optical coherence tomography. SLD, superluminescent diode; DC, directional coupler; C1, C2, microscope objectives; M, mirror; SXY, galvanometer scanning mirror head; MX, MY, scanner mirrors; L1, L2, lenses; PD, photodetector; ASO, analogue storage oscilloscope; TX, TY, triangle waveform generators.

 


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Figure 9. Spectra (left) and the temporal evolution (right) obtained with the set-up in Figure 8Go from a mirror as target [16]. If the beam is shifted by 3 mm from the rotation axis (bottom), higher frequency oscillations are generated, concentrated around a carrier, as shown in the left bottom figure [17]. a.u. represents arbitrary units for the strength of the photodetected signal.

 


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Figure 10. 3D display of in vivo optical-coherence tomography image of normal human skin from a volunteer's finger tip, [19]. Volume size: 5 mm x 4 mm x 1 mm (depth measured in air). The arrow ED shows the direction of exploration of the 3D reconstructed volume made from 40 en face slices acquired at 25 µm depth interval.

 


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Figure 11. (a) C-scan images of the fovea [20] obtained with the dual channel optical-coherence tomography (OCT)/confocal instrument in the C-scan imaging regimen. CSO: confocal scanning ophthalmoscopy image produced by the confocal channel; all the other images were provided by the OCT channel at depths shown below each image. Lateral size: 3 mm x 3 mm. (b) Large angle pair of images produced with the OCT/confocal instrument in the B-scan imaging regimen, showing the fovea and the optic nerve. 28° lateral size (peak to peak angular extension of the fan of scanned rays at the pupil). Top: CSO image; Bottom: B-scan OCT image (depth 2 mm in air). RNFL, retinal nerve fibre layer; PL, photoreceptor layer; RPE, retinal pigment epithelium [21]. Depth resolution in the OCT images: 12 µm.

 


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Figure 12. Simultaneous indocyanine green (ICG) (left) and en-face optical-coherence tomography (right) image from an healthy eye. Lateral size: 26°. Images collected at 40 s after ICG was released into the body [22]. RPE, retinal pigment epithelium.

 


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Figure 13. En face polarization sensitive optical-coherence tomography images of an extracted human tooth: linear output images of the V and H channels (top), net reflectivity and birefringence retardation images (bottom). The size of each image was 2 mm x 2 mm. The images were acquired from a depth corresponding to a 300 µm optical path in dental tissue and their thickness is less than 15 µm.

 





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