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British Journal of Radiology (2005) 78, 945-947
© 2005 British Institute of Radiology
doi: 10.1259/bjr/54004230

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Resazurin assay of radiation response in cultured cells

S Anoopkumar-Dukie, MSc1, J B Carey, BSc1, T Conere, MSc, PhD2, E O'Sullivan, BSc2, F N van Pelt, PhD1 and A Allshire, PhD1

1 Department of Pharmacology and Therapeutics, University College Cork, Cork and 2 Department of Medical Physics, Cork University Hospital, Wilton, Cork, Ireland



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Figure 1. (a) Resazurin reduction, (b) colony formation and (c) BrdU incorporation by HeLa cells 7 days (a, c) or 12 days (b) after irradiation at 2–8 Gy under normoxia ({square}) or hypoxia ({blacksquare}). Data are means±standard deviation for three independent experiments. OER, oxygen enhancement ratio calculated at 60% of unirradiated control values.

 





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