This Article Full Text (PDF) Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Guichard, M. Articles by Weeger, P. Search for Related Content PubMed PubMed Citation Articles by Guichard, M. Articles by Weeger, P.

Glutathione and cysteine levels in human tumour biopsies

M. Guichard, PhD F. Lespinasse, BS R. Estelin, MD ^* A. Gerbaulet, MD C. Haie, MD E. Lartigau, MD E. P. Malaise, MD, PhD C. Micheau, MD M. Prade, MD J. M. Richard, MD ^* and P. Weeger, MD ^*

Laboratoire de Radiobiologie Cellulaire (Unité Inserm 247), Institut Gustave-Roussy, 94805 Villejuif, France ^* Département de Chirurgie cervico-faciale, Institut Gustave-Roussy, 94805 Villejuif, France Service de Curiethérapie, Institut Gustave-Roussy, 94805 Villejuif, France Département de Pathologie (Histo A et B), Institut Gustave-Roussy, 94805 Villejuif, France

There is evidence that some human tumours could be treated with a combination of buthionine sulfoximine and hypoxic cell sensitizers. However, clinical application of this technique requires a prior knowledge of the level of non-protein bound sulfhydryl (NPSH) compounds in these tumours. The present study provides data on the levels of glutathione (GSH) and cysteine (CYS) in human tumour biopsies from the cervix and from the head and neck. The NPSH compounds were measured by high performance liquid chromatography. The median GSH values were 20.5 nmol/mg protein (cervix) and 23 nmol/mg protein (head and neck) while the median CYS values were 4.4 (cervix) and 4.2 nmol/mg protein (head and neck). The values varied widely from one patient to another.

Received for publication October 1, 1989. Revision received February 1, 1990.

This article has been cited by other articles:

				V. Vukovic, T. Nicklee, and D. W. Hedley Microregional Heterogeneity of Non-Protein Thiols in Cervical Carcinomas Assessed by Combined Use of HPLC and Fluorescence Image Analysis Clin. Cancer Res., May 1, 2000; 6(5): 1826 - 1832. [Abstract] [Full Text] [PDF]