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Measurement of plasma volume using ⁹⁹Tc^m, and ¹¹³In^m labelled proteins

Division of Nuclear Medicine, The Johns Hopkins Medical Institutions, Baltimore, Maryland, U.S.A.

This excerpt was created in the absence of an abstract.

The short-lived ¹³²I-labelled serum albumin (HSA) has previously been used for the repeated estimations of blood volume with reduced dose of radiation. The possibilities of using ⁹⁹Tc^m-HSA and ¹¹³In^m-chloride (the shortlived blood-pool scanning agents) for similar measurements have been explored. Simultaneous measurements of plasma volume were carried out in human subjects using ⁹⁹Tc^m-HSA or ¹¹³In^m-chloride with ¹³¹I-HSA. The values obtained with the new agents were comparatively higher (about 5 per cent) than results with ¹³¹I-HSA. Studies in dogs showed that a direct slow injection of ¹¹³In^mCl₃ in acidic saline could be used for the measurement of plasma volume, though pre-labelling of plasma transferrin by simple mixing improved the accuracy. The method was applied in repeated estimations of blood volume within a few hours in normal women and to determine changes in blood volume after delivery in pregnant women. The relative values of whole body radiation doses per estimation of plasma volume were calculated and found to be 0·16, 0·19, 0·28, 0·45, 2·30, 13·68 and 19·22 mrads respectively for proteins labelled with ⁹⁹Tc^m, ¹¹³In^m, ¹²³I, ¹³²I, ¹²⁵I, ¹³¹I and ⁵¹Cr.

Received for publication January 1, 1969.