Figure 2. Radiation-induced generation of ROS is abolished by preincubation with the antioxidant NAC, the AT₁RA losartan and the NADPH oxidase inhibitors DPI and apocynin. Rat brain microvascular endothelial cells were irradiated with 0–10 Gy -rays and ROS generation measured at 1 h post-irradiation using the oxidative-sensitive fluorescence probe H2DCFDA (A). In panels B and C, cells were pre-incubated with 5 mM NAC, 10 µM losartan, 10 µM DPI or apocynin for 30 min, respectively, prior to treatment with 10 Gy -rays. Mean±SE; n = 3; *p<0.05.