Figure 3. 49-year-old man with intrahepatic cholangiocarcinoma histologically diagnosed by needle biopsy and who underwent radiotherapy. (a) Grey scale fundamental ultrasound shows a hyperechoic lesion with hypoechoic periphery 6.4 cm in diameter, in S6 of the liver (arrows). (b) Contrast-enhanced ultrasound images by coded harmonic imaging at 45 s after administration of Levovist. Tumour vessels are observed only in tumour periphery (arrows). (c) Contrast-enhanced ultrasound images acquired from 1-s intermittent transmissions at 90 s after administration. Vascularity is homogeneously increased only in the tumour periphery (arrows). No vascular enhancement was evident within the tumour throughout the whole examination.