Figure 1. Time course of apoptosis induced by iodinated contrast media (ICM). (a) Representative flow cytometry profiles taken at 12 h culture of annexin v-fluorescein isothiocyanate (V-FITC) and propidium iodide staining of: (A), control; (B) iotrolan 20 mg I ml^-1; (C) iohexol 20 mg I ml^-1; (D) ioxaglate 20 mg I ml^-1; (E), diatrizoate 20 mg I ml^-1. The percentage given in the top left quadrant indicate the percentage of annexin V-FITC positive apoptotic neutrophils. (b) Results expressed as mean percentage of apoptotic cells±standard error of the mean. *, p<0.05 and , p<0.01 for ICM treated vs control neutrophils.